原文出处：

Shigeru Tanaka, Ken Ishii, Kazue Kasai, Sung Ok Yoon, and Yoshinaga Saeki

Neural Expression of G Protein-coupled Receptors GPR3, GPR6, and GPR12 Up-regulates Cyclic AMP Levels and Promotes Neurite Outgrowth
J. Biol. Chem. 2007 282: 10506-10515. First Published on February 6, 2007; doi:10.1074/jbc.M700911200 [Abstract] [Full Text] [PDF] [Supplemental Data]

相关基因：

GPR3

Official Symbol: GPR3 and Name: G protein-coupled receptor 3 [Homo sapiens]

Other Aliases: ACCA

Other Designations: OTTHUMP00000043209; adenylate cyclase constitutive activator

Chromosome: 1; Location: 1p36.1-p35

MIM: 600241

GeneID: 2827

GPR6

Official Symbol: GPR6 and Name: G protein-coupled receptor 6 [Homo sapiens]

Chromosome: 6; Location: 6q21

MIM: 600553

GeneID: 2830

GPR12

Official Symbol: GPR12 and Name: G protein-coupled receptor 12 [Homo sapiens]

Other Aliases: GPCR12, GPCR21, MGC138349, MGC138351

Chromosome: 13; Location: 13q12

MIM: 600752

GeneID: 2835

作者简介：

Yoshinaga Saeki, M.D., Ph.D.

Associate Professor
Department of Neurological Surgery

Ph.D.: Osaka University
Postdoctoral Training: Massachusetts General Hospital and Harvard Medical School

PHONE: (614) 292-3804
FAX: (614) 688-4882
E-MAIL: saeki.6@osu.edu

Link to NLM PubMed publications list for Yoshinaga Saeki (last 10 years)

Research Area:

• Gene- and cell-based therapy for neurological disorders
• Development and applications of viral vectors

Current Research:

My laboratory is developing therapeutic strategies for neurological disorders.  We are engaged in three major ongoing projects that employ multidisciplinary research techniques.

1. Developing and applying herpes simplex virus (HSV)-based amplicon vectors for gene therapy and neuroscience research; HSV amplicon vectors are plasmid-based, high-capacity vectors that have full HSV infection machinery.
   • identifying and characterizing cellular and immunological mechanisms that regulate HSV amplicon-mediated transgene expression
   • developing “indicator” HSV amplicon vectors to monitor various cellular activities
   • genetic engineering of neuronal cells using “regulatable” HSV amplicon vectors
2. Development and applications of engineered, oncolytic HSV vectors for brain tumor therapy.
   • identifying and characterizing cellular and immunological mechanisms that interfere with oncolytic activities of replication-conditional HSV vectors
   • developing novel oncolytic virotherapies that target brain tumor stem cells
3. Studying the roles of three related orphan G protein-coupled receptors, GPR3, GPR6, and GPR12, in the mammalian central nervous system
   • defining the functions of GPR3, GPR6, and GPR12 using knockout mice and cultured primary neurons
   • defining the roles of GPR3, GPR6, and GPR12 in neurological disorders, such as spinal cord injury and stroke

Techniques:

• Molecular biology:  cloning of genes; construction of viral vectors; cloning and engineering viral genomes; BAC engineering; ChIP-PCR; pulsed field-gel electrophoresis (PFGE); GFP and epitope tagging; and purification of His-tagged proteins.
• Cell Biology:  transfection of cultured cells; RNAi; immunocytochemistry; FACS analysis; immunoblotting; immunoprecipitation; and primary neural cultures.
• Imaging: confocal microscopic imaging; time-lapse fluorescent microscopic imaging; and in vivo bioluminescence imaging.
• Transgenic mice and small animal surgery: in vivo gene transfer.