Retroviral Infection Kit逆转录病毒感染试剂盒

Catalogue：CTK002  Storage: 4－8 ºC

Kit components:

Principle:

The MSCV (Murine Stem Cell Virus) Retroviral Expression System contains vectors that are optimized for introducing and expressing target genes in proliferating murine or human cell lines. This highly efficient system is ideal for analyzing gene expression and function in development, embryogenesis, and carcinogenesis in both cell culture and transgenic assays.

Designed for tough-to-transfect cells, the MSCV system contains two vectors: pMac-vGFP and helper that expresses protein elements required for producing high-titer virus. These vectors contain a specially designed long terminal repeat (LTR) from the murine stem cell virus that allows you to transduce most hard-to-transfect cell lines. This system drives high-level, constitutive expression of the target gene in most mammalian cell lines.

Step 1. Production of high-titer virus in 293 cells

1.       

Step 2. Infection of targeting cells

edr

Procedure illustration: