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PCR基础知识
免疫PCR要点
免疫PCR要点...
作者:发表于:2007-11-14 01:20:21 点击:104 评论:0 查阅全文...
免疫PCR基本实验步骤
免疫PCR的基本实验步骤...
作者:发表于:2007-11-14 01:18:55 点击:137 评论:0 查阅全文...
免疫PCR的种类
免疫PCR的种类...
作者:发表于:2007-11-14 01:17:07 点击:117 评论:0 查阅全文...
免疫PCR基本原理
免疫PCR(immuno polymerase chain reaction,IM-PCR)基本原理...
作者:发表于:2007-11-14 01:14:22 点击:212 评论:0 查阅全文...
Purification of PCR fragments for cloning
(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019 broe@ou.edu) After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder of the reaction is concentrated by...
作者:发表于:2007-08-20 09:46:12 点击:39 评论:0 查阅全文...
PCR 反应增强剂
DMSO and glycerol Recommended by a number of authors to improve amplification efficiency and specificity of PCR. However, in the multiplex reaction these enhancers gave conflicting results. For example, 5% DMSO improved the amplification of some pro...
作者:发表于:2006-08-18 09:34:04 点击:87 评论:0 查阅全文...
PCR产物量小或没有目的产物
Possible Causes: Components Primer concentration is too low. Primer concentrations not balanced. Make sure primers are present in equal concentrations. New primers are required. Some primers are immune to optimization. See Primer Design. Nested prim...
作者:发表于:2006-08-18 09:31:18 点击:90 评论:0 查阅全文...
PCR产生非特异条带
Possible Causes: Components 可能原因之一反应组分 Primer concentration is too high. Primer degeneracy is too high. Nested primers are required. New primers are required. Some primers are immune to optimization and it's possible that your primers are...
作者:发表于:2006-08-18 09:30:36 点击:145 评论:0 查阅全文...