- Inverse PCR
- Inverse PCR (IPCR), described by Ochman et al in 1988, is a method for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in t...
- 作者：发表于：2008-03-22 15:02:19 点击：41 评论：0 查阅全文...
- Yeast Colony PCR--酵母菌落PCR
- This method is more reliable than the old method of adding yeast directly to PCR tubes. No more than 1 microliter of the crude DNA should be added to the 50 microliter PCR reaction because the SDS in the DNA prep can inhibit the PCR reaction. Note: T...
- 作者：发表于：2008-03-22 14:59:10 点击：24 评论：0 查阅全文...
- Expand High Fidelity PCR--高保真PCR
- Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20 cycles, expect ~8% of the products will have at least one mutation. See Boehringer data sheet...
- 作者：发表于：2008-03-22 14:57:47 点击：11 评论：0 查阅全文...
- Single Cell PCR
- Single Cell PCR...
- 作者：发表于：2008-03-22 14:55:26 点击：11 评论：0 查阅全文...
- PCR-SSCP技术
- 实验原理 聚合酶链式反应-单链构象多态（Polymerase Chain Reaction-Single Strand Conformation Polymorphism，PCR-SSCP）技术是在PCR技术基础上发展起来的，它是一种简单、快速、经济的用来显示在PCR反应产物中单碱基突变（点突变）的手段。该方法已被用做癌基因和抑...
- 作者：发表于：2008-03-01 11:36:50 点击：94 评论：0 查阅全文...
- TAIL-PCR(Termal Asymmetric Interlaced PCR)
- 很早就有用随机引物的PCR，但由于无法有效地控制由随机引物引发的非特异产物的产生，所以一直未能广泛应用。近年来由IJiu等设计的TAIL-PCR(Termal Asymmetric Interlaced PCR)又叫热不对称交错PCR，则解决了这个问题，后来有研究表明，经改良过的TAIL-PCR成功地从突变...
- 作者：发表于：2008-02-12 03:01:31 点击：213 评论：0 查阅全文...
- A Problem of TAIL PCR
- Dear fellow Arabidopsis researchers I am going to use TAIL PCR for amplification of genomic sequences flanking the T-DNA insertion. I would like to use the methode described in the following source: Liu et al., Plant Journal 8: 457-463 (1995) In thi...
- 作者：发表于：2008-02-12 03:00:40 点击：68 评论：0 查阅全文...
- High-throughput TAIL-PCR as a tool to identify DNA flanking insertions
- Thermalasymmetricinterlacedpolymerasechainreaction(TAIL-PCR)isafastandefficientmethodtoamplifyunknownsequencesadjacenttoknowninsertionsitesinArabidopsis. Nested,insertion-specificprimersareusedtogetherwitharbitrarydegenerateprimers(ADprimers),whicha...
- 作者：发表于：2008-02-12 02:59:23 点击：78 评论：0 查阅全文...
- 栏目列表
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热门关注
- RACE-PCR克隆基因的几点建议
- RACE PCR简介
- 什么是TAIL-PCR?
- 什么是反向PCR（inverse PCR
- TAIL-PCR(Termal Asymmetric
- SSCP检测SNP原理，步骤及常
- 反转录差异显示技术(DDRT-PC
- 差异显示技术(DD-PCR)及其应
- 荧光PCR原理
- 定量PCR Taqman探针设计要领
- 基因拼接--SOE法和SDL法
- 定量PCR Taqman探针试剂之选
- PCR-SSCP技术
- Thermal Asymmetric Interla
- High-throughput TAIL-PCR a
- RAPD技术应用中的一些问题及
- A Problem of TAIL PCR
- 荧光PCR/实时定量PCR技术应
- 荧光定量PCR技术简介
- RAPD技术在昆虫学中的应用
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