- 甲基化PCR Methylation-Specific PCR（MSP）
- 甲基化PCR Methylation-Specific PCR（MSP）...
- 作者：发表于：2008-11-13 17:41:09 点击：2 评论：0 查阅全文...
- Amplified Fragment Length Polymorphism (AFLP)
- The AFLP (Amplified Fragment Length Polymorphism) technique is used to visualize hundreds of amplified DNA bands of restriction fragments simultaneously (Vos et al., 1995)....
- 作者：发表于：2008-07-20 23:55:29 点击：14 评论：0 查阅全文...
- Random Amplified Polymorphic DNA (RAPD)
- Random Amplified Polymorphic DNA (RAPD)...
- 作者：发表于：2008-07-20 23:50:01 点击：21 评论：0 查阅全文...
- Inverse PCR
- Inverse PCR (IPCR), described by Ochman et al in 1988, is a method for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in t...
- 作者：发表于：2008-03-22 15:02:19 点击：105 评论：0 查阅全文...
- Yeast Colony PCR--酵母菌落PCR
- This method is more reliable than the old method of adding yeast directly to PCR tubes. No more than 1 microliter of the crude DNA should be added to the 50 microliter PCR reaction because the SDS in the DNA prep can inhibit the PCR reaction. Note: T...
- 作者：发表于：2008-03-22 14:59:10 点击：143 评论：0 查阅全文...
- Expand High Fidelity PCR--高保真PCR
- Expand is a mixture of Taq and Pwo DNA polymerases (Boehringer). Pwo polymerase has 3' to 5' exo proofreading activity. If amplifying a 1 Kb fragment for 20 cycles, expect ~8% of the products will have at least one mutation. See Boehringer data sheet...
- 作者：发表于：2008-03-22 14:57:47 点击：34 评论：0 查阅全文...
- Single Cell PCR
- Single Cell PCR...
- 作者：发表于：2008-03-22 14:55:26 点击：32 评论：0 查阅全文...
- PCR-SSCP技术
- 实验原理 聚合酶链式反应-单链构象多态（Polymerase Chain Reaction-Single Strand Conformation Polymorphism，PCR-SSCP）技术是在PCR技术基础上发展起来的，它是一种简单、快速、经济的用来显示在PCR反应产物中单碱基突变（点突变）的手段。该方法已被用做癌基因和抑...
- 作者：发表于：2008-03-01 11:36:50 点击：254 评论：0 查阅全文...
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