- A Problem of TAIL PCR
- Dear fellow Arabidopsis researchers I am going to use TAIL PCR for amplification of genomic sequences flanking the T-DNA insertion. I would like to use the methode described in the following source: Liu et al., Plant Journal 8: 457-463 (1995) In thi...
- 作者：发表于：2008-02-12 03:00:40 点击：113 评论：0 查阅全文...
- High-throughput TAIL-PCR as a tool to identify DNA flanking insertions
- Thermalasymmetricinterlacedpolymerasechainreaction(TAIL-PCR)isafastandefficientmethodtoamplifyunknownsequencesadjacenttoknowninsertionsitesinArabidopsis. Nested,insertion-specificprimersareusedtogetherwitharbitrarydegenerateprimers(ADprimers),whicha...
- 作者：发表于：2008-02-12 02:59:23 点击：120 评论：0 查阅全文...
- Thermal Asymmetric Interlaced Tail PCR
- Purpose: Mu TAIL PCR produces a population of fragments with one end anchored in Robertson's Mutator (Mu) TIR's and the other in the Mu flanking DNA. These products may be used for cloning, gel analysis, or as hybridization probes. This protocol de...
- 作者：发表于：2008-02-12 02:52:14 点击：153 评论：0 查阅全文...
- Quick DNA Prep - Used for TAIL PCR
- Quick DNA Prep - Used for TAIL PCR 1. Excise 4-10 green leaves from mature plant and collect in a 1.5 ml microfuge tube. 2. Macerate tissue with plastic mini pestle. 3. Add 200 l extraction buffer (200 mM Tris pH 7.5, 250 mM NaCl, 25 mM EDTA pH 8.0,...
- 作者：发表于：2008-02-12 02:51:29 点击：48 评论：0 查阅全文...
- TAIL-PCR to clone flanking sequence from Feldmanns lines
- The first thing you need to do before starting your TAIL-PCR is to figure out which border of the T-DNA is flanking the plant genome, especially with Ken Feldmann's lines since the concatameric nature of the T-DNA inserts. The best way to do that is...
- 作者：发表于：2008-02-12 02:33:47 点击：62 评论：0 查阅全文...
- 基因拼接--SOE法和SDL法
- 基因拼接--SOE法和SDL法...
- 作者：发表于：2007-10-13 02:25:35 点击：128 评论：1 查阅全文...
- RAPD技术在昆虫学中的应用
- 随机扩增多态DNA(Random Amplified Polymorphic DNA，简称RAPD)是J．Williams和J．Welsh两个研究小组于1990年同时提出的一种随机引物扩增，寻找多态DNA片段的遗传标记技术...
- 作者：发表于：2007-09-14 07:17:03 点击：80 评论：0 查阅全文...
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