参考文献
1. Bhagwat, A. A., R. P. Phadke, D. Wheeler, S. Kalantre, M. Gudipati, and M.
Bhagwat. 2003. Computational methods and evaluation of RNA stabilization reagents for genome-wide expression studies. J. Microbiol. Methods 55:399–409.
2. Bremer, H., and P. P. Dennis. 1996. Modulation of chemical composition and other parameters of the cell by growth rate, p. 1553–1569. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B.Magasanik, W. S. Reznikoff, M. Riley, M. Schaecter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
3. Bustin, S. A. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25:169–193.
4. Freeman, W. M., S. J. Walker, and K. E. Vrana. 1999. Quantitative RT-PCR:
pitfalls and potential. BioTechniques 26:112–125.
5. Gourse, R. L., T. Gaal, M. S. Bartlett, J. A. Appleman, and W. Ross. 1996. rRNA transcription and growth rate-dependent regulation of ribosome synthesis in Escherichia coli. Annu. Rev. Microbiol. 50:645–677.
6. Keer, J. T., and L. Birch. 2003. Molecular methods for the assessment of bacterial viability. J. Microbiol. Methods 53:175–183.
7. Kingombe, C., G. Huys, M. Tonolla, M. Albert, J. Swings, R. Peduzzi, and T.
Jemmi. 1999. PCR detection, characterization, and distribution of virulence genes in Aeromonas spp. Appl. Environ. Microbiol. 65:5293–5302.
8. Kjeldgaard, N. O., and K. Gausing. 1974. Regulation of biosynthesis of ribosomes, p. 369–392. In M. Nomura, A. Tissie`res, and P. Lengyel (ed.), Ribosomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
9. Malorny, B., J. Hoorfar, C. Bunge, and R. Helmuth. 2003. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl. Environ. Microbiol. 69:290–296.
10. Matthews, J., M. Chung, and J. Matyas. 2002. Persistent DNA contamination in competitive RT-PCR using cRNA internal standards: identity, quantity, and control. BioTechniques 32:1412–1417.
11. McClelland, M., K. E. Sanderson, J. Spieth, S. W. Clifton, P. Latreille, L. Courtney, S. Porwollik, J. Ali, M. Dante, F. Du, S. Hou, D. Layman, S. Leonard, C. Nguyen, K. Scott, A. Holmes, N. Grewal, E. Mulvaney, E. Ryan, H. Sun, L. Florea, W. Miller, T. Stoneking, M. Nhan, R. Waterston, and R. K. Wilson. 2001. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 413:852–856.
12. Neretin, L. N., A. Schippers, A. Pernthaler, K. Hamann, R. Amann, and B. B. Jorgensen. 2003. Quantification of dissimilatory (bi)sulphite reductase gene expression in Desulfobacterium autotrophicum using real-time RT-PCR. Environ. Microbiol. 5:660–671.
13. Paton, A., and J. Paton. 1998. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J. Clin. Microbiol. 36:598–602.
14. Pfaffl, M. W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45. [Online.]
15. Pfaffl, M. W., and M. Hageleit. 2001. Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR. Biotechnol. Lett. 23:275–282.
16. Rodriguez-Lazaro, D., M. Hernandez, T. Esteve, J. Hoorfar, and M. Pla. 2003. A rapid and direct real time PCR-based method for identification of Salmonella spp. J. Microbiol. Methods 54:381–390.
17. Rokbi, B., D. Seguin, B. Guy, V. Mazarin, E. Vidor, F. Mion, M. Cadoz, and M.-J. Quentin-Millet. 2001. Assessment of Helicobacter pylori gene expression within mouse and human gastric mucosae by real-time reverse transcriptase PCR. Infect. Immun. 69:4759–4766.
18. Ruimy, R., V. Breittmayer, V. Boivin, and R. Christen. 1994. Assessment of the state of activity of individual bacterial cells by hybridization with a ribosomal RNA targeted fluorescently labelled oligonucleotidic probe. FEMS Microbiol. Ecol.. 15:207–213.
19. Sharma, V. K., E. A. Dean-Nystrom, and T. A. Casey. 1999. Semi-automated fluorogenic PCR assays (TacMan) for rapid detection of Escherichia coli O157:H7 and other Shiga toxigenic E. coli. Mol. Cell. Probes 13:291–302.
20. Sheridan, G. E. C., C. I. Masters, J. A. Shallcross, and B. M. Mackey. 1998. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl. Environ. Microbiol. 64:1313–1318.
21. Straub, T. M., and D. P. Chandler. 2003. Towards a unified system for detecting waterborne pathogens. J. Microbiol. Methods 53:185–197. 
22. Vandecasteele, S. J., W. E. Peetermans, R. Merckx, and J. Van Eldere. 2001. Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman quantitative PCR during in vitro growth and under different conditions. J. Bacteriol. 183:7094–7101.
23. Vandecasteele, S. J., W. E. Peetermans, R. Merckx, M. Van Ranst, and J. Van Eldere. 2002. Use of gDNA as internal standard for gene expression in staphylococci in vitro and in vivo. Biochem. Biophys. Res. Commun. 291: 528–534.
24. Walker, N. J. 2002. A technique whose time has come. Science 296:557–559.
25. Wawrik, B., J. H. Paul, and F. R. Tabita. 2002. Real-time PCR quantification of rbcL (ribulose-1,5-bisphosphate carboxylase/oxygenase) mRNA in diatoms and pelagophytes. Appl. Environ. Microbiol. 68:3771–3779.
26. Weinbauer, M. G., I. Fritz, D. F. Wenderoth, and M. G. Ho¨fle. 2002. Simultaneous extraction from bacterioplankton of total RNA and DNA suitable for quantitative structure and function analyses. Appl. Environ. Microbiol. 68:1082–1087.
27. Weinbauer, M. G., and M. G. Ho¨fle. 2001. Quantification of nucleic acids from aquatic environments by using green-fluorescent dyes and microtiter plates, p. 2.1.3–2.1.10. In A. Akkermans, J. van Elsas, and F. de Bruijn (ed.), Molecular microbial ecology manual, 5th suppl. Kluwer Academic Publishers, Dordrecht, The Netherlands.
28. World Health Organization. 2002. The world health report. Reducing risks, promoting healthy life. World Health Organization, Geneva, Switzerland. 
29. Zhang, J., and C. D. Byrne. 1999. Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR. Biochem. J. 337:231– 241.