热门关键字:  生物  基因  细胞  动物  蛋白
Western Blotting
绿色荧光蛋白基因(GFP)的免疫印迹
绿色荧光蛋白基因(GFP)的免疫印迹...
作者:发表于:2008-08-03 19:05:14 点击:59 评论:0 查阅全文...
Western blotting Protocol
Western blotting Protocol...
作者:发表于:2008-08-03 19:01:36 点击:50 评论:0 查阅全文...
组织印迹的Western杂交
组织印迹的Western杂交...
作者:发表于:2008-08-03 19:01:10 点击:11 评论:0 查阅全文...
Too many bands on a Western blot
Too many bands on a Western blot...
作者:发表于:2008-08-03 19:00:35 点击:9 评论:0 查阅全文...
Western-Blot操作流程
试剂配制 (一)母液 1.0mol/L Tris·HCl Tris (MW121.14) 30.29g 蒸馏水 200ml 溶解后,用浓盐酸调pH至所需点(见下所示),最后用蒸馏水定容至250ml,高温灭菌后室温下保存。 PH HCl 7.4 约17ml 7.5 约16m 7.6 约15ml 8.0 约10ml 1.74mg/ml (10mmol/L)PMSF P...
作者:发表于:2008-08-03 10:57:39 点击:60 评论:0 查阅全文...
Western Blot的原理和所用试剂汇总
Western Blot的原理和所用试剂汇总...
作者:发表于:2008-08-03 18:44:25 点击:99 评论:0 查阅全文...
Western印迹法
Western印迹法...
作者:发表于:2008-08-03 18:40:31 点击:80 评论:0 查阅全文...
Western Blotting - Antibodies
1. Load gel in apparatus as described by manufacturer. 2. Transfer proteins according to above ensuring that the NCM is toward the positive electrode. When using the Hoefer apparatus be sure to either precool the buffer and run the unit in a cold ro...
作者:发表于:2008-08-03 18:39:21 点击:17 评论:0 查阅全文...